Paper
14 February 2018 A novel fiber-free technique for brain activity imaging in multiple freely behaving mice
Shigenori Inagaki, Masakazu Agetsuma, Takeharu Nagai
Author Affiliations +
Abstract
Brain functions and related psychiatric disorders have been investigated by recording electrophysiological field potential. When recording it, a conventional method requires fiber-based apparatus connected to the brain, which however hampers the simultaneous measurement in multiple animals (e.g. by a tangle of fibers). Here, we propose a fiber-free recording technique in conjunction with a ratiometric bioluminescent voltage indicator. Our method allows investigation of electrophysiological filed potential dynamics in multiple freely behaving animals simultaneously over a long time period. Therefore, this fiber-free technique opens up the way to investigate a new mechanism of brain function that governs social behaviors and animal-to-animal interaction.
© (2018) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Shigenori Inagaki, Masakazu Agetsuma, and Takeharu Nagai "A novel fiber-free technique for brain activity imaging in multiple freely behaving mice", Proc. SPIE 10482, Optogenetics and Optical Manipulation 2018, 104820I (14 February 2018); https://doi.org/10.1117/12.2302396
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KEYWORDS
Brain

Signal detection

Bioluminescence

Cranial windows

Neuroimaging

Image intensifiers

Imaging systems

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