Photoacoustic microscopy of single cells employing an intensity-modulated diode laser

Gregor Langer; Bianca Buchegger; Jaroslaw Jacak; Manoj Kumar Dasa; Thomas A. Klar; Thomas Berer

doi:10.1117/12.2289978

19 February 2018 Photoacoustic microscopy of single cells employing an intensity-modulated diode laser

Gregor Langer, Bianca Buchegger, Jaroslaw Jacak, Manoj Kumar Dasa, Thomas A. Klar, Thomas Berer

Author Affiliations +

Proceedings Volume 10494, Photons Plus Ultrasound: Imaging and Sensing 2018; 104942L (2018) https://doi.org/10.1117/12.2289978
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

In this work, we employ frequency-domain photoacoustic microscopy to obtain photoacoustic images of labeled and unlabeled cells. The photoacoustic microscope is based on an intensity-modulated diode laser in combination with a focused piezo-composite transducer and allows imaging of labeled cells without severe photo-bleaching. We demonstrate that frequency-domain photoacoustic microscopy realized with a diode laser is capable of recording photoacoustic images of single cells with sub-µm resolution. As examples, we present images of undyed human red blood cells, stained human epithelial cells, and stained yeast cells.

Citation Download Citation

Gregor Langer, Bianca Buchegger, Jaroslaw Jacak, Manoj Kumar Dasa, Thomas A. Klar, and Thomas Berer "Photoacoustic microscopy of single cells employing an intensity-modulated diode laser", Proc. SPIE 10494, Photons Plus Ultrasound: Imaging and Sensing 2018, 104942L (19 February 2018); https://doi.org/10.1117/12.2289978

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

;

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE