Infrared Studies Of Biomolecules In Water Using Wavelength Modulated Derivative Spectroscopy

Barry H. Dorfman

doi:10.1117/12.951645

8 May 1989 Infrared Studies Of Biomolecules In Water Using Wavelength Modulated Derivative Spectroscopy

Barry H. Dorfman

Proceedings Volume 1057, Biomolecular Spectroscopy; (1989) https://doi.org/10.1117/12.951645
Event: OE/LASE '89, 1989, Los Angeles, CA, United States

Abstract

Many problems in cancer research deal with the functional change in a biomolecule due to an alteration in its structure or conformation. Such changes can come about from ligand binding, drug intercalation, radiation and/or chemical damage, or from changes in the molecule's environment. In many cases, such a small change in the structure or conformation of the molecule can have far reaching consequences. It is important, therefore, to develop research tools capable of studying small differences in conformations and/or small alterations in molecules of biological importance in aqueous media. We are proposing to apply wavelength modulation derivative spectroscopy, a high sensitivity method used in Condensed Matter Physics, to the study of small structural changes in biomolecules. Preliminary studies suggest that such infrared spectroscopy can be performed on biomolecules dissolved or dispersed in aqueous media, which overcomes a major obstacle - the need to work with nonaqueous solvents - in the application of infrared spectroscopy to the study of biomolecules.

Citation Download Citation

Barry H. Dorfman "Infrared Studies Of Biomolecules In Water Using Wavelength Modulated Derivative Spectroscopy", Proc. SPIE 1057, Biomolecular Spectroscopy, (8 May 1989); https://doi.org/10.1117/12.951645

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