Presentation
4 March 2019 Immune response of corneal epithelial cells in response to methicillin-resistant Staphylococcus aureus (MRSA) and rose bengal photodynamic antimicrobial therapy (PDAT) (Conference Presentation)
Heather A. Durkee, Jorge Maestre-Mesa, Alejandro Arboleda, Mariela C. Aguilar, Guillermo Amescua, Jean-Marie Parel, Darlene Miller
Author Affiliations +
Abstract
Introduction: The prevalence and diversity of methicillin-resistant Staphylococcus aureus (MRSA) virulent factors as well as the host inflammatory response makes managing MRSA keratitis challenging. Alternative treatments are being investigated that can both neutralize the MRSA toxins while also reducing the host immune response. One such alternative is Photodynamic Antimicrobial Therapy (PDAT); however, limited research has been performed to understand the impact of PDAT on the host immune response during MRSA-related infections. Purpose: To measure the immune response (Interleukin(IL)-1, 6 and 8) of human corneal epithelial cells in an ex-vivo tissue model following MRSA infection and rose bengal PDAT. Methods: EpiCorneal tissue models (Maktek) were prepared according to manufacturer’s protocol. All groups were tested in duplicate: Control, Infection, Infection-PDAT. Infections were created in the tissue with three separate MRSA inocula (10E5 CFU/mL). For the Infection-PDAT group, 0.1% rose bengal solution was applied and wells were irradiated with a custom-made green LED light source. After 30 minutes, fluid from all of the tissue model wells was collected and IL-1, 6 and 8 were quantified with ELISA kits (Thermo Fisher Scientific). Results: The EpiCorneal Tissue model simulated the immune response of human corneal epithelial cells during MRSA infection and treatment with PDAT. Compared to the control, the immune response increased in all three MRSA infection groups. Of the cytokines tested, IL-8 showed the greatest response in the tissue models, followed by IL-6 and IL-1. In the Infection-PDAT groups, immune response was mixed depending on the MRSA strain. The largest downregulation of immune factors was observed in the community-associated MRSA strain.
Conference Presentation
© (2019) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Heather A. Durkee, Jorge Maestre-Mesa, Alejandro Arboleda, Mariela C. Aguilar, Guillermo Amescua, Jean-Marie Parel, and Darlene Miller "Immune response of corneal epithelial cells in response to methicillin-resistant Staphylococcus aureus (MRSA) and rose bengal photodynamic antimicrobial therapy (PDAT) (Conference Presentation)", Proc. SPIE 10863, Photonic Diagnosis and Treatment of Infections and Inflammatory Diseases II, 108630O (4 March 2019); https://doi.org/10.1117/12.2511992
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KEYWORDS
Photodynamic therapy

Tissues

Green light emitting diodes

Light sources

Manufacturing

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