Sub-wavelength convertible Bessel-beam and Gaussian-beam photoacoustic microscope in reflection-mode for in-vivo application

Byullee Park; Hoyong Lee; Seungwan Jeon; Joongho Ahn; Hyung Ham Kim; Chulhong Kim

doi:10.1117/12.2506562

27 February 2019 Sub-wavelength convertible Bessel-beam and Gaussian-beam photoacoustic microscope in reflection-mode for in-vivo application

Byullee Park, Hoyong Lee, Seungwan Jeon, Joongho Ahn, Hyung Ham Kim, Chulhong Kim

Author Affiliations +

Proceedings Volume 10878, Photons Plus Ultrasound: Imaging and Sensing 2019; 1087869 (2019) https://doi.org/10.1117/12.2506562
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

A sub-wavelength convertible Bessel-beam (BB) and Gaussian-beam (GB) photoacoustic microscope (PAM) in reflection-mode has been developed. A miniature ultrasonic transducer was placed in front of the objective with a high numerical aperture and a working distance of a few millimeters to achieve the reflection-mode and sub-wavelength resolution. For BB-PAM system, a conical lens and an achromatic doublet lens were used to achieve extended depth of field (DoF). In particular, it was designed to easily convert the system from BB to GB by removing the two lenses described above, so that the DoF of BB- and GB-PAM can be compared accurately. The 532 nm pulsed laser used in this system was coupled to a single mode fiber. The sample was scanned using X-Y direction motors and the system was controlled using Labview software. The lateral resolution of the focus regions of BB- and GB-PAM obtained were 300 and 260 nm, respectively. As a result of measuring the DoF of BB-PAM, it was about 250 ~ 300 μm, which was about 7 ~ 8 times better than the DoF of GB-PAM. In-vivo vascular structure of a mouse ear was successfully visualized using BB- and GB-PAM to compare the DoF of the two systems. Thus, the system developed in this study confirmed that BBPAM enabled high-resolution imaging at extended DoF than GB-PAM, and further believed that this system could be useful for a variety of biomedical research.

Citation Download Citation

Byullee Park, Hoyong Lee, Seungwan Jeon, Joongho Ahn, Hyung Ham Kim, and Chulhong Kim "Sub-wavelength convertible Bessel-beam and Gaussian-beam photoacoustic microscope in reflection-mode for in-vivo application", Proc. SPIE 10878, Photons Plus Ultrasound: Imaging and Sensing 2019, 1087869 (27 February 2019); https://doi.org/10.1117/12.2506562

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
5 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

In vivo imaging

Imaging systems

Photoacoustic microscopy

Photoacoustic spectroscopy

Image resolution

Signal detection

Carbon

Show All Keywords

Keywords/Phrases

Search In:

Publication Years