Broadband hyperspectral stimulated Raman scattering microscopy with a parabolic fiber amplifier source

Benjamin Figueroa; Walter Fu; Tai Nguyen; Kseniya Shin; Bryce Manifold; Frank Wise; Dan Fu

doi:10.1117/12.2508104

4 March 2019 Broadband hyperspectral stimulated Raman scattering microscopy with a parabolic fiber amplifier source

Benjamin Figueroa, Walter Fu, Tai Nguyen, Kseniya Shin, Bryce Manifold, Frank Wise, Dan Fu

Proceedings Volume 10889, High-Speed Biomedical Imaging and Spectroscopy IV; 1088910 (2019) https://doi.org/10.1117/12.2508104
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Hyperspectral Stimulated Raman scattering (hsSRS) microscopy has recently emerged as a powerful non-destructive technique for label-free chemical imaging of biological samples. In most hsSRS imaging experiments, the SRS spectral range is limited by the total bandwidth of the excitation laser to ~300 cm^-1 and spectral resolution of ~25 cm^-1. Here we present a novel approach for broadband hsSRS microscopy based on parabolic fiber amplification to provide linearly chirped broadened Stokes pulses. This novel hsSRS instrument provides >600 cm^-1 spectral coverage and ~10 cm^-1 spectral resolution. We further demonstrated broadband hsSRS imaging of the entire Raman fingerprint region for resolving distribution of major biomolecules in fixed cells. Moreover, we applied broadband hsSRS in imaging amyloid plaques in human brain tissue with Alzheimer’s disease.

Citation Download Citation

Benjamin Figueroa, Walter Fu, Tai Nguyen, Kseniya Shin, Bryce Manifold, Frank Wise, and Dan Fu "Broadband hyperspectral stimulated Raman scattering microscopy with a parabolic fiber amplifier source", Proc. SPIE 10889, High-Speed Biomedical Imaging and Spectroscopy IV, 1088910 (4 March 2019); https://doi.org/10.1117/12.2508104

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