Rapid and multiplexed detection of Lyme disease using a grating coupled-fluorescent plasmonics (GC-FP) biosensor platform

Eunice Chou; Arturo Pilar; Ernest F Guignon; William Page; Yi-Pin Lin; Nathaniel C. Cady

doi:10.1117/12.2507973

7 March 2019 Rapid and multiplexed detection of Lyme disease using a grating coupled-fluorescent plasmonics (GC-FP) biosensor platform

Eunice Chou, Arturo Pilar, Ernest F Guignon, William Page, Yi-Pin Lin, Nathaniel C. Cady

Author Affiliations +

Proceedings Volume 10895, Frontiers in Biological Detection: From Nanosensors to Systems XI; 108950C (2019) https://doi.org/10.1117/12.2507973
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Infection with the spirochete Borrelia burgdorferi leads to Lyme disease (LD), the most prevalent tick-borne illness in the Northern Hemisphere. If left untreated, the infection spreads throughout the body, causing multisystem disease. The current standard for LD diagnosis is a two-tiered approach (ELISA followed by Western blot), which targets the immune response to bacterial proteins. This approach, however, lacks sensitivity and specificity, leading to misdiagnosis. We developed a protein microarray assay to detect antibodies against B. burgdorferi proteins with high sensitivity using grating-coupled surface plasmon resonance, combined with fluorescence imaging (Grating Coupled-Fluorescent Plasmonics, GC-FP). Here, we use GC-FP for rapid and multiplexed detection of antibodies from B. burgdorferi in human serum. We confirmed the fluorescence enhancement capability of GC-FP analysis and optimized reagent concentrations for detection of serum antibodies present in human LD. By conducting GC-FP analysis of patient serum samples, we were able to accurately diagnose LD in patients with disseminated and early-stage infection. Our results show that GC-FP can detect IgG antibodies in highly dilute human sera (up to 1:1250X serum dilution) and we are currently establishing whether or not our GC-FP platform can detect serum antibodies with greater sensitivity and specificity compared to the standard Western blot approach. Altogether, our work provides a potential path towards replacement of the cumbersome two-tiered testing algorithm, and thus a streamlined approach to LD diagnosis.

Conference Presentation

Citation Download Citation

Eunice Chou, Arturo Pilar, Ernest F Guignon, William Page, Yi-Pin Lin, and Nathaniel C. Cady "Rapid and multiplexed detection of Lyme disease using a grating coupled-fluorescent plasmonics (GC-FP) biosensor platform", Proc. SPIE 10895, Frontiers in Biological Detection: From Nanosensors to Systems XI, 108950C (7 March 2019); https://doi.org/10.1117/12.2507973

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