In vivo detection of microRNA within plants using plasmonic nanosensors

Bridget M. Crawford; Pietro Strobbia; Hsin-Neng Wang; Rodolfo Zentella; Maxim I. Boyanov; Zhen-Ming Pei; Tai-Ping Sun; Kenneth M. Kemner; Tuan Vo-Dinh

doi:10.1117/12.2529818

9 September 2019 In vivo detection of microRNA within plants using plasmonic nanosensors

Bridget M. Crawford, Pietro Strobbia, Hsin-Neng Wang, Rodolfo Zentella, Maxim I. Boyanov, Zhen-Ming Pei, Tai-Ping Sun, Kenneth M. Kemner, Tuan Vo-Dinh

Author Affiliations +

Proceedings Volume 11082, Plasmonics: Design, Materials, Fabrication, Characterization, and Applications XVII; 1108215 (2019) https://doi.org/10.1117/12.2529818
Event: SPIE Nanoscience + Engineering, 2019, San Diego, California, United States

Abstract

Gene expression monitoring within whole plants is critical for many applications ranging from plant biology to biofuel development. Herein, we report a unique multimodal method for in vivo imaging and biosensing of nucleic acid biotargets, specifically microRNA, within whole plant leaves by integrating three complementary techniques: surface-enhanced Raman scattering (SERS), X-ray fluorescence (XRF), and plasmonics-enhanced two-photon luminescence (TPL). The method described utilizes plasmonic nanostar-based inverse molecular sentinel (iMS) nanoprobes, which not only provide large Raman signal enhancement upon target binding, but also allow for localization and quantification by XRF and plasmonics-enhanced TPL. This report lays the foundation for the use of plasmonic nanoprobes for in vivo functional imaging of nucleic acid biotargets in whole plants.

Conference Presentation

Citation Download Citation

Bridget M. Crawford, Pietro Strobbia, Hsin-Neng Wang, Rodolfo Zentella, Maxim I. Boyanov, Zhen-Ming Pei, Tai-Ping Sun, Kenneth M. Kemner, and Tuan Vo-Dinh "In vivo detection of microRNA within plants using plasmonic nanosensors", Proc. SPIE 11082, Plasmonics: Design, Materials, Fabrication, Characterization, and Applications XVII, 1108215 (9 September 2019); https://doi.org/10.1117/12.2529818

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