Alkyne-tag SERS imaging for visualizing small molecule drugs in live cells

Kota Koike; Kazuki Bando; Jun Ando; Nicholas Smith; Kosuke Dodo; Satoshi Kawata; Mikiko Sodeoka; Katsumasa Fujita

doi:10.1117/12.2545872

19 February 2020 Alkyne-tag SERS imaging for visualizing small molecule drugs in live cells

Kota Koike, Kazuki Bando, Jun Ando, Nicholas Smith, Kosuke Dodo, Satoshi Kawata, Mikiko Sodeoka, Katsumasa Fujita

Proceedings Volume 11219, Visualizing and Quantifying Drug Distribution in Tissue IV; 112190D (2020) https://doi.org/10.1117/12.2545872
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Raman microscopy is a powerful tool to observe molecular distribution in live cells. Here, we propose a technique to detect small molecule drugs using alkyne-tag surface-enhanced Raman scattering (SERS) imaging. To obtain SERS effect, we use gold nanoparticles as SERS probes so that we can enhance Raman scattering of small molecule drugs at low concentration with several orders of magnitude. We use alkyne tag to selectively detect the drug molecules by using the Raman peak of alkyne in the spectral silent region. Home-built slit-scanning Raman microscopy enables us to perform rapid SERS imaging. We successfully detect SERS signal from an alkyne-tagged inhibitor of a lysosomal enzyme with gold nanoparticle modified glass substrate.

Citation Download Citation

Kota Koike, Kazuki Bando, Jun Ando, Nicholas Smith, Kosuke Dodo, Satoshi Kawata, Mikiko Sodeoka, and Katsumasa Fujita "Alkyne-tag SERS imaging for visualizing small molecule drugs in live cells", Proc. SPIE 11219, Visualizing and Quantifying Drug Distribution in Tissue IV, 112190D (19 February 2020); https://doi.org/10.1117/12.2545872

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available