Shining a light on antibiotic selection: optimised live/dead fluorescence spectrometry for rapid antimicrobial susceptibility testing (Conference Presentation)

Julia Robertson; Fang Ou; Cushla M. McGoverin; Frédérique Vanholsbeeck; Simon Swift

doi:10.1117/12.2544082

10 March 2020 Shining a light on antibiotic selection: optimised live/dead fluorescence spectrometry for rapid antimicrobial susceptibility testing (Conference Presentation)

Julia Robertson, Fang Ou, Cushla M. McGoverin, Frédérique Vanholsbeeck, Simon Swift

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Proceedings Volume 11223, Photonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2020; 112230E (2020) https://doi.org/10.1117/12.2544082
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Antibiotic resistance is a serious threat to public health. The empiric use of the wrong antibiotic occurs due to urgency in treatment combined with slow, culture-based diagnostic techniques. Inappropriate antibiotic choice can promote the development of antibiotic resistance. We investigated live/dead spectrometry using a fluorimeter (Optrode) as a rapid alternative to culture-based techniques through application of the LIVE/DEAD® BacLightTM Bacterial Viability Kit. Killing was detected by the Optrode in near real-time when E. coli was treated with ampicillin and stained with SYTO 9 and/or PI but only when a suitable concentration, bacterial growth phase, and treatment time was used.

Conference Presentation

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Julia Robertson, Fang Ou, Cushla M. McGoverin, Frédérique Vanholsbeeck, and Simon Swift "Shining a light on antibiotic selection: optimised live/dead fluorescence spectrometry for rapid antimicrobial susceptibility testing (Conference Presentation)", Proc. SPIE 11223, Photonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2020, 112230E (10 March 2020); https://doi.org/10.1117/12.2544082

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KEYWORDS

Spectroscopy

Fluorescence spectroscopy

Luminescence

Resistance

Bacteria

Calibration

Diagnostics

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