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9 March 2020 Investigation of in vitro human iPSC-derived neuronal networks using holographic stimulation (Conference Presentation)
F. Schmieder, R. Habibey, V. Busskamp, L. Büttner, J. W. Czarske
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Proceedings Volume 11227, Optogenetics and Optical Manipulation 2020; 112270D (2020) https://doi.org/10.1117/12.2546288
Event: SPIE BiOS, 2020, San Francisco, California, United States
Abstract
Optogenetics is a valuable tool set for the investigation of neuronal connections. Data on the connectome of human neurons is indispensable e.g. for the understanding of human neurodegenerative diseases. Providing this data for in vitro human stem-cell derived neuronal networks is the aim of our research. We use a holographic illumination setup with up to 1.7 kHz frame rate, 8 µm spatial resolution and 31 mm working distance for the stimulation of neurons expressing Channelrhodopsin2. In this work, we will present the measured functional connectivity of single neurons we retrieve using spatially resolved stimulation and simultaneous extracellular multi electrode recording.
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F. Schmieder, R. Habibey, V. Busskamp, L. Büttner, and J. W. Czarske "Investigation of in vitro human iPSC-derived neuronal networks using holographic stimulation (Conference Presentation)", Proc. SPIE 11227, Optogenetics and Optical Manipulation 2020, 112270D (9 March 2020); https://doi.org/10.1117/12.2546288
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KEYWORDS
Holography
In vitro testing
Electrodes
Neurons
Neural networks
Animal model studies
Optogenetics
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