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The aims of this study were to clarify the influences of fat layer thickness (FLT) and aerobic capacity on optical property dynamics during exercise by using near-infrared time-resolved spectroscopy (NIRTRS) in humans. Endurance-trained men and healthy control men with various FLT performed a ramp incremental cycling exercise test until exhaustion. Absorption coefficient, reduced scattering coefficient, and mean optical pathlength in the vastus lateralis were continuously monitored by three-wavelength (763, 801 and 836 nm) NIRTRS. Our results have demonstrated that FLT and aerobic capacity affect the optical property dynamics during exercise and that the effects may differ depending on the wavelength.
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