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There is a fundamental limitation in optical resolution photoacoustic microscopy (OR-PAM) systems, as optical resolution may only be maintained to a depth equal to the transport mean free path of the excitation laser. Here we present a non-contact OR-PAM that can break this barrier. Monte-Carlo simulations and experiments in tissue simulating phantoms validate that the proposed method is capable of imaging at over 2.5mm deep achieving micron resolution, and greater than 5mm at lower resolutions.
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Benjamin R. Ecclestone, Amirmohammad Farzaneh, Kevan Bell, Parsin H. Reza, "Deep optical resolution photoacoustic remote sensing microscopy (Conference Presentation)," Proc. SPIE 11240, Photons Plus Ultrasound: Imaging and Sensing 2020, 1124013 (20 April 2020); https://doi.org/10.1117/12.2546765