Epi-mode tomographic quantitative phase imaging in thick scattering samples

Francisco E. Robles

doi:10.1117/12.2547693

20 February 2020 Epi-mode tomographic quantitative phase imaging in thick scattering samples

Francisco E. Robles

Author Affiliations +

Proceedings Volume 11251, Label-free Biomedical Imaging and Sensing (LBIS) 2020; 112511Y (2020) https://doi.org/10.1117/12.2547693
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Quantitative phase imaging (QPI) is an important tool in biomedicine that yields label-free access to cellular and subcellular structures with nanometer-scale sensitivity. However, implementation of QPI involves a transmissionbased geometry and requires thin samples, severely hindering its overall utility in biomedicine. Here we describe our recently developed method, quantitative oblique back illumination microscopy (qOBM), which overcomes this significant limitation and achieves epi-mode, tomographic, quantitative phase imaging in thick samples, including intact thick tissues. Here we describe the method in detail, show validation experiments and results from thick scattering samples.

Citation Download Citation

Francisco E. Robles "Epi-mode tomographic quantitative phase imaging in thick scattering samples", Proc. SPIE 11251, Label-free Biomedical Imaging and Sensing (LBIS) 2020, 112511Y (20 February 2020); https://doi.org/10.1117/12.2547693

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