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Paper performs results of SERS-active surfaces fabrication for Raman bacterial cells analysis. Based on FDTD simulation, the synthesis of colloidal gold nanoparticles (NPs) with the size range of 10 – 100 nm has been performed by the following methods: a) femtosecond laser ablation of a plate in a liquid; b) chemical reduction from tetrachloroauric acid trihydrate (HAuCl4). Optimal sizes and shapes of the particles with a maximum of plasmon absorption in the range 500 – 800 nm have been determined by numerical simulation. For NPs deposited on quartz glass with rodamine 6G (R6G) and E. Coli bacterial cells, SERS solutions have been tested.
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Andrey Y. Zyubin, Karina I. Matveeva, I. I. Kon, D. S. Kalinnikov, M. A. Shevchenko, Ilia G. Samusev, "New method of SERS-active gold surfaces fabrication for bacterial cells Raman analysis," Proc. SPIE 11345, Nanophotonics VIII, 113452I (1 April 2020); https://doi.org/10.1117/12.2555895