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Laser ablation (LA) is a minimally invasive procedure based on light/tissue interaction aimed to induce a controlled tumor necrosis by increasing tissue temperature. Given the relationship between tissue damage and produced heat, LA needs a fine control of evolving thermal effects in order to evaluate and control procedure outcome. This study relies on biomedical optics principles for non-invasive diagnostic tools development, and presents a contactless approach based on hyperspectral imaging (HSI) to monitor thermal damage during in vivo porcine LA. By collecting relative pixel-by-pixel reflectance/absorbance of a wide range spectrum (500-1000nm), HSI can track molecular structure modifications caused by the thermoablative procedure. Indeed, these modifications alter tissue light scattering and absorption. In order to investigate tissue spectrum change by increasing temperature, HSI was collected at fixed maximum temperatures (37, 60, 70, 80, 90, 100, 110 °C) and immediately after LA (1, 2, 3, 4, and 5 minutes). Tissue spectral response for two tests was analyzed also relying on the ablated area considered. Regions of Interest of different dimensions (16, 77, and 170 pixels) were placed in the images after applying a motion correction. Obtained spectra show noticeable variations once a specific temperature threshold has been reached (80-100 °C). Specifically, the measured absorbance variation for selected wavelengths (630, 760, 960nm, for methemoglobin, deoxyhemoglobin, and water respectively) confirms tissue optical behavior dependence with its thermal state. This preliminary investigation discloses the potential of HSI measurement to characterize LA damage, encouraging future studies to standardize this novel technique.
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