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5 March 2021 Fluorescence lifetime imaging and spectroscopy for enhanced protoporphyrin IX guided brain tumor surgery
David Reichert, Mikael T. Erkkilä, Georg Widhalm, Barbara Kiesel, Lisa Wadiura, Petra Mercea, Marco Andreana, Angelika Unterhuber, Marco Wilzbach, Christoph Hauger, Wolfgang Drexler, Rainer Leitgeb, Thomas Rötzer, Adelheid Wöhrer
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Proceedings Volume 11629, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics; 116290B (2021) https://doi.org/10.1117/12.2578660
Event: SPIE BiOS, 2021, Online Only
Abstract
Protoporphyrin IX (PpIX) fluorescence-guided surgery has established as a standard for resecting malign glioma. However, low-grade glioma or sparsely infiltrated brain often emit weak PpIX fluorescence and are hard to distinguish from non-pathological tissue. Furthermore, spectrally overlapping autofluorescence inherently limits the sensitivity of fluorescence-intensity based PpIX detection. We therefore integrated frequency-domain fluorescence lifetime imaging together with a spectrometer in a surgical microscope. When analyzing human glioma samples ex vivo, weak PpIX fluorescence could be differentiated from the autofluorescence background through increased lifetimes. Characteristic peaks in the spectral measurements (635, 705nm) confirmed low concentrations of PpIX in the tissue.
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David Reichert, Mikael T. Erkkilä, Georg Widhalm, Barbara Kiesel, Lisa Wadiura, Petra Mercea, Marco Andreana, Angelika Unterhuber, Marco Wilzbach, Christoph Hauger, Wolfgang Drexler, Rainer Leitgeb, Thomas Rötzer, and Adelheid Wöhrer "Fluorescence lifetime imaging and spectroscopy for enhanced protoporphyrin IX guided brain tumor surgery", Proc. SPIE 11629, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics, 116290B (5 March 2021); https://doi.org/10.1117/12.2578660
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KEYWORDS
Fluorescence lifetime imaging
Brain
Luminescence
Tumors
Surgery
Fluorescence spectroscopy
Spectroscopy
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