Rapid, accurate intraoperative assessment of excised specimens and in vivo resection sites is a critical need in tissue-conserving cancer surgeries. We develop a multimodal imaging system integrating ultraviolet photoacoustic remote sensing (UV-PARS) microscopy with spectral-domain optical coherence tomography (SD-OCT) to provide comprehensive, co-registered information for identifying atypical tissue features. 1050-nm SD-OCT generates a widefield, depth-resolved view of subsurface tissue morphology, with 9-μm axial resolution. UV-PARS produces virtual histological C-scan images of cell nuclei, at 390-nm lateral resolution. Dual-contrast in vivo mouse imaging, and ex vivo imaging of breast and prostate specimens is demonstrated, and correlated with conventional H and E staining.
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