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5 March 2021 Burst-mode multispectral optoacoustic microscopy for large-scale dermatologic imaging
Urs Alexander Tassilo Hofmann, Weiye Li, Johannes Rebling, Héctor Estrada, Pavel V. Subochev, Daniel Razansky
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Proceedings Volume 11642, Photons Plus Ultrasound: Imaging and Sensing 2021; 116422Z (2021) https://doi.org/10.1117/12.2578316
Event: SPIE BiOS, 2021, Online Only
Abstract
We present a fast, multispectral acoustic resolution optoacoustic microscope using a new burst-mode triggering scheme. Three pulsed laser sources are combined to retrieve spectral images across large field-of-views extending over 25mm by 25mm at 28μm lateral and 14μm axial resolution with an overfly scan of a few minutes. Highly sensitive PVDF transducer allows detection of structures 3.8mm below the human skin surface with per pulse energies of only 25μJ. The newly developed system overcomes limitations of previously reported scanning optoacoustic microscopy and mesoscopy implementations, offering a major leap forward in terms of clinical usability, laser safety, effective penetration depth and spectral unmixing capabilities.
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Urs Alexander Tassilo Hofmann, Weiye Li, Johannes Rebling, Héctor Estrada, Pavel V. Subochev, and Daniel Razansky "Burst-mode multispectral optoacoustic microscopy for large-scale dermatologic imaging", Proc. SPIE 11642, Photons Plus Ultrasound: Imaging and Sensing 2021, 116422Z (5 March 2021); https://doi.org/10.1117/12.2578316
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KEYWORDS
Optoacoustics
Microscopy
Multispectral imaging
Optical microscopy
Organisms
Skin
Spatial resolution
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