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Polarized nonlinear scanning imaging using two photon fluorescence, harmonic generation or coherent Raman has been extensively developed to image molecular orientational organization in tissues. Current approaches using fast modulation (electro-optic) are however still limited to seconds time scales due to their required voltage shape modulation. To reach higher frequencies, we have developed an interferometric frequency shift-based method using Acousto-Optic Modulation. We present degree-precision performances in the retrieved information even at low signal to noise level and sub-second dynamics. The method is applied to real time actin structural imaging in cells as well as collagen and lipids organization imaging in tissues.
Miguel Sison andSophie Brasselet
"Real-time imaging of molecular organization by point scanning nonlinear fast polarization modulation", Proc. SPIE 11646, Polarized Light and Optical Angular Momentum for Biomedical Diagnostics, 116460Q (5 March 2021); https://doi.org/10.1117/12.2578547
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Miguel Sison, Sophie Brasselet, "Real-time imaging of molecular organization by point scanning nonlinear fast polarization modulation," Proc. SPIE 11646, Polarized Light and Optical Angular Momentum for Biomedical Diagnostics, 116460Q (5 March 2021); https://doi.org/10.1117/12.2578547