label-free super-resolution imaging through second-harmonic generation structured illumination microscopy

Andreas Zheng; Nansen Zhou; Renjie Zhou

doi:10.1117/12.2578579

5 March 2021 label-free super-resolution imaging through second-harmonic generation structured illumination microscopy

Andreas Zheng, Nansen Zhou, Renjie Zhou

Proceedings Volume 11653, Quantitative Phase Imaging VII; 116531N (2021) https://doi.org/10.1117/12.2578579
Event: SPIE BiOS, 2021, Online Only

Conference Poster

Abstract

We propose a new label-free widefield super-resolution imaging technique which involves structured illumination and second harmonic generation (SHG). In an interferometric setup with a reference beam and a sample beam, the use of structured light effectively doubles the region of detectable spatial frequencies. This procedure utilizes both, intensity and phase information of the sample. By solving the wave equations for SHG fields, the images can then be reconstructed in Fourier space with a spatial resolution up to four times the Abbe diffraction limit.

Conference Presentation

Citation Download Citation

Andreas Zheng, Nansen Zhou, and Renjie Zhou "label-free super-resolution imaging through second-harmonic generation structured illumination microscopy", Proc. SPIE 11653, Quantitative Phase Imaging VII, 116531N (5 March 2021); https://doi.org/10.1117/12.2578579

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