Non-invasive micromechanical sensing and real-time 3D imaging of in vitro biological systems.

Tania Mendonca; Andrew B. Matheson; Katarzyna Lis-Slimak; Akosua Anane-Adjei; Cameron Alexander; Cathy L. R. Merry; Lynn Paterson; Manlio Tassieri; Paul Dalgarno; Amanda J. Wright

doi:10.1117/12.2595357

1 August 2021 Non-invasive micromechanical sensing and real-time 3D imaging of in vitro biological systems.

Tania Mendonca, Andrew B. Matheson, Katarzyna Lis-Slimak, Akosua Anane-Adjei, Cameron Alexander, Cathy L. R. Merry, Lynn Paterson, Manlio Tassieri, Paul Dalgarno, Amanda J. Wright

Author Affiliations +

Proceedings Volume 11798, Optical Trapping and Optical Micromanipulation XVIII; 117980C (2021) https://doi.org/10.1117/12.2595357
Event: SPIE Nanoscience + Engineering, 2021, San Diego, California, United States

Abstract

We present a bespoke new instrument for micro-mechanical sensing and imaging of 3D cell cultures. Our instrument combines optical tweezers and multiplane imaging for 3D optical microrheology, with light sheet microscopy for 3D fluorescence imaging. This integrated system allows us to visualise cells in their 3D environment while also mapping the physical properties of the extracellular matrix local to the cells. Such a holistic approach will allow for a wide range of fundamental questions in the life sciences to be addressed by effectively sensing the micro-world from a cell’s perspective.

Conference Presentation

Citation Download Citation

Tania Mendonca, Andrew B. Matheson, Katarzyna Lis-Slimak, Akosua Anane-Adjei, Cameron Alexander, Cathy L. R. Merry, Lynn Paterson, Manlio Tassieri, Paul Dalgarno, and Amanda J. Wright "Non-invasive micromechanical sensing and real-time 3D imaging of in vitro biological systems.", Proc. SPIE 11798, Optical Trapping and Optical Micromanipulation XVIII, 117980C (1 August 2021); https://doi.org/10.1117/12.2595357

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available