In this work, a simple and effective sensor using single-mode fiber (SMF) tapered structure is developed to detect different concentrations of acetylcholine solutions, and its function is to test the probe's performance. A layer of synthesized gold nanoparticles (AuNPs) and zinc oxide nanoparticles (ZnO-NPs) is used to immobilize this type of SMF-based tapered structure. The work is based on the well-known phenomenon of localized surface plasmon resonance (LSPR) principle. A tapered region of the probe with a high fraction power of evanescent wave can stimulate LSPR and produce specific absorption peaks sensitive to the refractive index variation. The sensor probes performance was examined, including their stability, repeatability, reusability, and selectivity. Furthermore, the biosensor's ability to improve performance was tested in the experiment.
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