Low-damage and large scale optical condensation of useful bacteria with bubble-mimetic substrate

Kota Hayashi; Mamoru Tamura; Shiho Tokonami; Takuya Iida

doi:10.1117/12.2616125

27 October 2021 Low-damage and large scale optical condensation of useful bacteria with bubble-mimetic substrate

Kota Hayashi, Mamoru Tamura, Shiho Tokonami, Takuya Iida

Author Affiliations +

Proceedings Volume 11926, Optical Manipulation and Structured Materials Conference 2021; 119260N (2021) https://doi.org/10.1117/12.2616125
Event: SPIE Technologies and Applications of Structured Light, 2021, Online, Japan

Abstract

We have demonstrated low-damage optical condensation by developing an unconventional substrate with controlled spatial configuration of photothermal source. This substrate was called as “bubble-mimetic substrate”, in which, a solid microsphere with the same size as light-induced sub-millimeter bubble was chemically fixed on the substrate and coated with metallic thin film. Under CW laser irradiation onto the metallic thin film on the top of the solid microsphere, several tens thousands of useful bacteria dispersed in liquid were assembled around the solid microsphere with survival rate higher than 95 %. Such a high survival rate was maintained even if laser power was increased to be approximately 60 mW. Our obtained result will expand the application range of optical condensation in biological science.

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Kota Hayashi, Mamoru Tamura, Shiho Tokonami, and Takuya Iida "Low-damage and large scale optical condensation of useful bacteria with bubble-mimetic substrate", Proc. SPIE 11926, Optical Manipulation and Structured Materials Conference 2021, 119260N (27 October 2021); https://doi.org/10.1117/12.2616125

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