Extended-wavelength diffuse reflectance spectroscopy (EWDRS) combines two spectrometers extending the wavelength range up to 1500 nm, which provides more information than visible or near-infrared DRS alone and indicates the improved ability to differentiate biological tissues. Identification of Neurovascular bundles (NVB) is a main challenging during surgery, while current EWDRS studies have been prove the ability in open surgeries. However, theoretical simulations for complex multilayer structure in EWDRS range have yet to be reported. Monte Carlo (MC) model has been developed to accurately simulate the random propagations in complex and multilayered structures in various optical applications. We report the design and development of an EWDRS system with a fiber optic EWDRS probe. In addition, 2-layer tissue simulating phantoms with different top-layer thickness are developed and MC simulations of EWDRS spectra based on the optical properties of phantoms are compared against empirical measurements, demonstrating the accuracy of MC model in simulating multilayers structures and a superficial bias in probe sampling volume. Finally, measurements taken during dissection of the NVB in an ex vivo chicken thigh animal model are reported and confirm the ability of EWDRS to identify peripheral NVB from adjacent tissues. The results showed the developed phantoms had the ability to mimic blood content and lipid absorption features in visible and near-infrared region and simulated spectra had same tendency as measured spectra. Additionally, the classification results from the animal model displayed the overall accuracy was over 92%, which indicated the feasibility of identification of NVB from adjacent tissues.
|