Multicolor stimulated Raman scattering microscopy and its applications

Yasuyuki Ozeki

doi:10.1117/12.2615280

2 March 2022 Multicolor stimulated Raman scattering microscopy and its applications

Yasuyuki Ozeki

Proceedings Volume 11957, Biomedical Vibrational Spectroscopy 2022: Advances in Research and Industry; 1195709 (2022) https://doi.org/10.1117/12.2615280
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Stimulated Raman scattering (SRS) microscopy allows for sensitive molecular vibrational imaging and is opening up a variety of biomedical applications. To fully utilize the capability of SRS, we developed a multicolor SRS microscope, which is capable of acquiring SRS images at the video rate, while the wavenumber can be changed in a frame-by-frame manner. Also our system is integrated with a confocal fluorescence microscope. I will introduce various vibrational imaging applications such as label-free imaging, supermultiplex imaging, metabolic imaging, and so on. We also describe functional Raman probes, which will further broaden the applications of SRS microscopy.

Conference Presentation

Citation Download Citation

Yasuyuki Ozeki "Multicolor stimulated Raman scattering microscopy and its applications", Proc. SPIE 11957, Biomedical Vibrational Spectroscopy 2022: Advances in Research and Industry, 1195709 (2 March 2022); https://doi.org/10.1117/12.2615280

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