Widely tunable near-infrared Raman fiber laser irradiating breast cancer cells leads to cell size reduction and increased granularity of intracellular components as a precursor to cell death

Santosh Aparanji; Siya Kamat

doi:10.1117/12.2610027

2 March 2022 Widely tunable near-infrared Raman fiber laser irradiating breast cancer cells leads to cell size reduction and increased granularity of intracellular components as a precursor to cell death

Santosh Aparanji, Siya Kamat

Author Affiliations +

Proceedings Volume 11958, Optical Interactions with Tissue and Cells XXXIII; and Advanced Photonics in Urology; 1195809 (2022) https://doi.org/10.1117/12.2610027
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Photodynamic therapy and radiotherapy have traditionally relied on fixed wavelength sources (typically 1064 nm Nd:YAG laser), due to their ready availability. But with advances in development of wide wavelength spanning Raman fiber lasers, a greater flexibility in choice of the light source is provided. In a previous work by our group, we demonstrated the first use of a widely tunable Raman fiber laser (from 1-1.5 um) in achieving varying degrees of cytotoxicity with wavelength at fixed power and exposure for an epithelial breast cancer cell line. However, the reasons for this variation in cytotoxicity was unknown. In this work, we analyse the causes of this variation of cytotoxicity with wavelength for the same epithelial breast cancer cells irradiated in-vitro. Cell-viability is performed through a Propidium-Iodide based assay analysed through a flow cytometer. Thereafter, with the implementation of a novel-image processing algorithm on the same cytometer plots, we obtain a surprising insight into the reasons for cell death for certain wavelengths of irradiation. Specifically, we hypothesise that the effect of these wavelengths is twofold: to desiccate the water content of the cells, leading to reduction in cell size; and to lead to increased granularity or denaturation in the intra-cellular components. We make a surprising observation that cell size reduction is not as correlated with cytotoxicity as increased granularity. This paves the way for a better and insightful deployment of tunable wavelength Raman lasers in photodynamic therapy with the choice of an optimum wavelength to achieve the greatest tumoricidal effect.

Citation Download Citation

Santosh Aparanji and Siya Kamat "Widely tunable near-infrared Raman fiber laser irradiating breast cancer cells leads to cell size reduction and increased granularity of intracellular components as a precursor to cell death", Proc. SPIE 11958, Optical Interactions with Tissue and Cells XXXIII; and Advanced Photonics in Urology, 1195809 (2 March 2022); https://doi.org/10.1117/12.2610027

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
7 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Raman spectroscopy

Cell death

Fiber lasers

Radiotherapy

Cancer

Tunable lasers

Breast cancer

Show All Keywords

Keywords/Phrases

Search In:

Publication Years