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Microarray chip is a highly sensitive and high-throughput genetic detection technology based on the principle of complementary pairing of nucleic acid sequence bases and using polymerase chain reaction (PCR), and its chip structure and material have a great impact on the detection. Therefore, a new type of PCR chip is designed. The large multi-physical field simulation software Comsol is used to simulate the coupling of thermodynamics and structural mechanics of the designed chip. Finite element simulation is used to experimentally optimize the chip material selection in practice, find the composite material (cyclic olefin copolymer (COC)) that has the least effect on signal processing and does not require polar treatment of the chip substrate, and design the optimal chip structure. Three kinds of standard chips (internal reference, negative and positive) were prepared by using FAM fluorescent probe. Positive luminescent spots were randomly labeled for detection. This detection is used to evaluate the value of practical application of this chip. The results show that the maximum deformation displacement of the chip is 0.6 mm at 4–100℃, which is instantaneously irradiated by a 1 KW radiation light source, and the transmittance of the model calculated by the penetrated radiation power is 83.7%. In practical application, several materials are irradiated with excitation light to detect their excitation effect, and COC has the smallest excitation effect and the lowest background signal value. A chip containing 203 microarray spots was prepared according to the above conclusions, and the detection results were 100% consistent.
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