Single cell detection using self-mixing interferometry based on microfluidic chip

Menglei Zhang; Yu Zhao; Jiawei Li; Chen Tao

doi:10.1117/12.2604497

24 November 2021 Single cell detection using self-mixing interferometry based on microfluidic chip

Menglei Zhang, Yu Zhao, Jiawei Li, Chen Tao

Proceedings Volume 12067, AOPC 2021: Biomedical Optics; 1206706 (2021) https://doi.org/10.1117/12.2604497
Event: Applied Optics and Photonics China 2021, 2021, Beijing, China

Abstract

Laser self-mixing interferometry (SMI) is often used for displacement, vibration, and velocity measurement. At present, the measurement accuracy has reached tens of nanometers, but it has not been used for single cell detection. In this research, a microfluidic chip-based equipment using SMI technology for label-free single cell detection was demonstrated. The detection experiments were performed to verify chicken erythrocytes and human breast cancer cells T47D. In order to better analyze these data, the Hilbert transform was used to convert the time domain signal into phase information. It is found that there are more fringes in the signal of larger breast cancer cells. The power spectrum of the signal shows that the velocity of the cell is positively correlated with the Doppler shift. The new method for single cell detection proposed in this paper provides a new idea for real-time cell detection.

Citation Download Citation

Menglei Zhang, Yu Zhao, Jiawei Li, and Chen Tao "Single cell detection using self-mixing interferometry based on microfluidic chip", Proc. SPIE 12067, AOPC 2021: Biomedical Optics, 1206706 (24 November 2021); https://doi.org/10.1117/12.2604497

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