Three-dimensional positioning and imaging through scattering media based on the double-helix point spread function

Jingjing Gao; Honglin Liu; Shensheng Han

doi:10.1117/12.2617682

15 February 2022 Three-dimensional positioning and imaging through scattering media based on the double-helix point spread function

Jingjing Gao, Honglin Liu, Shensheng Han

Author Affiliations +

Proceedings Volume 12166, Seventh Asia Pacific Conference on Optics Manufacture and 2021 International Forum of Young Scientists on Advanced Optical Manufacturing (APCOM and YSAOM 2021); 121665Q (2022) https://doi.org/10.1117/12.2617682
Event: Seventh Asia Pacific Conference on Optics Manufacture and 2021 International Forum of Young Scientists on Advanced Optical Manufacturing (APCOM and YSAOM 2021), 2021, Hong Kong, Hong Kong

Abstract

Double-helix point spread function (DH-PSF), which can convert the axial position of an object into double-spot rotation, is widely used in single-molecule localization microscopy due to its excellent axial localization ability. However, the current single-molecule localization microscopy techniques based on the DH-PSF are mostly applied in non-scattering or weak scattering environments, and are only applicable to sparse objects. In this paper, we study the three-dimensional positioning and imaging through scattering media based on the DH-PSF. The image of the object can be reconstructed from the recorded speckle pattern via speckle autocorrelation and phase retrieval techniques, and the three-dimensional coordinate information of the object can be obtained by the rotation angle and the center of the double spots modulated by the DH-PSF. The positioning accuracy in axial direction for non-sparse objects in simulation is better than 10μm, which is greatly improved compared with our previous work (Journal of Optics, 2021, 23(2):025602 (5pp)). This method has many potential applications in acquiring dynamical information of the micro-object motion.

Citation Download Citation

Jingjing Gao, Honglin Liu, and Shensheng Han "Three-dimensional positioning and imaging through scattering media based on the double-helix point spread function", Proc. SPIE 12166, Seventh Asia Pacific Conference on Optics Manufacture and 2021 International Forum of Young Scientists on Advanced Optical Manufacturing (APCOM and YSAOM 2021), 121665Q (15 February 2022); https://doi.org/10.1117/12.2617682

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KEYWORDS

Scattering

Light scattering

Point spread functions

Imaging systems

Scattering media

Speckle

3D image processing

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