Obtaining antibodies to silymarin using the adjuvant properties of gold nanoparticles and their application to study the uptake of silymarin into cells

Lev A. Dykman; Sergey A. Staroverov; Alexander S. Fomin; Konstantin P. Gabalov

doi:10.1117/12.2625711

29 April 2022 Obtaining antibodies to silymarin using the adjuvant properties of gold nanoparticles and their application to study the uptake of silymarin into cells

Lev A. Dykman, Sergey A. Staroverov, Alexander S. Fomin, Konstantin P. Gabalov

Author Affiliations +

Proceedings Volume 12194, Computational Biophysics and Nanobiophotonics; 121940Y (2022) https://doi.org/10.1117/12.2625711
Event: XXV Annual Conference Saratov Fall Meeting 2021; and IX Symposium on Optics and Biophotonics, 2021, Saratov, Russian Federation

Abstract

A complex of gold nanoparticles with silymarin, a flavonoid-type hepatoprotector isolated from the fruits of milk thistle (Silybum marianum L. Gaertn), was obtained by the method of green synthesis. The resulting conjugate was used to immunize rabbits. After the completion of immunization, the isolated serum immunoglobulins were additionally purified using the method of high performance liquid chromatography. Purified anti-silymarin IgGs were tested for specific antigen binding by dot immunoassay. The detection limit for silymarin was 12.5 ng. The obtained antibodies were labeled with TRITC and used to study the penetration of silymarin into the cells of the reticuloendothelial system by immunofluorescence microscopy. It was demonstrated that silymarin conjugated with gold nanoparticles penetrated into peritoneal macrophages much more efficiently as compared to the native preparation. The results obtained can serve as a basis for creating a new dosage form of silymarin in the form of its conjugate with gold nanoparticles, which enhances hepatoprotective properties due to better penetration of the drug into the cells of the reticuloendothelial system.

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Lev A. Dykman, Sergey A. Staroverov, Alexander S. Fomin, and Konstantin P. Gabalov "Obtaining antibodies to silymarin using the adjuvant properties of gold nanoparticles and their application to study the uptake of silymarin into cells", Proc. SPIE 12194, Computational Biophysics and Nanobiophotonics, 121940Y (29 April 2022); https://doi.org/10.1117/12.2625711

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KEYWORDS

Nanoparticles

Gold

Microscopy

Luminescence

Liver

Magnesium

Proteins

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