Efficient Fourier ptychographic microscopy with hybrid coherent and incoherent illumination

Yao Fan; Jiasong Sun; Qian Chen; Chao Zuo

doi:10.1117/12.2643979

30 December 2022 Efficient Fourier ptychographic microscopy with hybrid coherent and incoherent illumination

Yao Fan, Jiasong Sun, Qian Chen, Chao Zuo

Proceedings Volume 12316, Advanced Optical Imaging Technologies V; 123160D (2022) https://doi.org/10.1117/12.2643979
Event: SPIE/COS Photonics Asia, 2022, Online Only

Conference Poster

Abstract

Fourier ptychographic microscopy (FPM) is a promising high-throughput imaging technique for biological research, medical research, and pathological diagnosis. Based on varying angle illumination and synthetic aperture, it achieves the imaging resolution beyond the diffraction limit of objective lens across a wide field-of-view (FOV). In FPM, sufficient redundant data is required to ensure the convergence of iteration. However, excessive acquisition and reconstruction time cost lead to the main limitations of FPM in imaging efficiency. In this paper, we propose efficient FPM (EFPM) that distinguishes between bright and dark field illuminations, to reduce the number of images collected by Fourier strobe imaging. In bright-field acquisition, we adopt partially coherent illumination to record a single image, reducing the accumulated time of multiple LED sequential illumination to a single exposure. In addition, sparse sampling is used in the dark-field images acquisition to significantly reduce the number of captured images. Thus, EFPM yields imaging resolution reaching 3NAobj using only 7 images. The experiment on USAF resolution sample is presented to demonstrate that EFPM achieves high resolution and wide FOV through significantly reduced data, providing a rapid means for pathological research.

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Yao Fan, Jiasong Sun, Qian Chen, and Chao Zuo "Efficient Fourier ptychographic microscopy with hybrid coherent and incoherent illumination", Proc. SPIE 12316, Advanced Optical Imaging Technologies V, 123160D (30 December 2022); https://doi.org/10.1117/12.2643979

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