Multispectral Singlet Oxygen Luminescent Dosimetry (MSOLD) for PHOTOFRIN-mediated photodynamic therapy

Weibing Yang; Vivek Rastogi; Hongjing Sun; Dvij Sharma; Brian C. Wilson; Robert H. Hadfield; Timothy C. Zhu

doi:10.1117/12.2652590

14 March 2023 Multispectral Singlet Oxygen Luminescent Dosimetry (MSOLD) for PHOTOFRIN-mediated photodynamic therapy

Weibing Yang, Vivek Rastogi, Hongjing Sun, Dvij Sharma, Brian C. Wilson, Robert H. Hadfield, Timothy C. Zhu

Proceedings Volume 12359, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXXI; 1235908 (2023) https://doi.org/10.1117/12.2652590
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Direct detection of singlet-state oxygen ([¹O₂]) constitutes the holy grail dosimetric method for type II PDT, a goal that can be quantified using multispectral singlet oxygen dosimetry (MSOLD). However, the short lifetime and extremely weak nature of the singlet oxygen signal produced has given rise to a need to improve MSOLD signal-to-noise ratio. This study examines methods for optimizing MSOLD signal acquisition, specifically employing an orthogonal arrangement between detection and PDT treatment light, consisting of two fiber optics - connected to a 632-nm laser and an InGaAs detector respectively. Light collected by the InGaAs detector is then passed through a filter wheel, where spectral emission measurements are taken at 1200 nm, 1240 nm, 1250 nm, 1270 nm, and 1300 nm. The data, after fitting to the fluorescence background and a gaussian-fit for the singlet oxygen peak, is established for the background-subtracted singlet oxygen emission signal. The MSOLD signal is then compared with the singlet oxygen explicit dosimetry (SOED) results, based on direct measurements of in-vivo light fluence (rate), in-vivo Photofrin concentration, and tissue oxygenation concentration. This study focuses on validating the sensitivity and minimum detectability of MSOLD signal in various in-vitro conditions. Finally, the MSOLD device will be tested in Photofrin-mediated PDT for mice bearing Radiation- Induced Fibrosarcoma (RIF) tumors.

Conference Presentation

Citation Download Citation

Weibing Yang, Vivek Rastogi, Hongjing Sun, Dvij Sharma, Brian C. Wilson, Robert H. Hadfield, and Timothy C. Zhu "Multispectral Singlet Oxygen Luminescent Dosimetry (MSOLD) for PHOTOFRIN-mediated photodynamic therapy", Proc. SPIE 12359, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXXI, 1235908 (14 March 2023); https://doi.org/10.1117/12.2652590

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