Simultaneous 2-photon and 3-photon multimodal signal acquisition for multiphoton microscopy

Wentao Wu; Christoph Brandt; Shuo Tang

doi:10.1117/12.2649196

25 April 2023 Simultaneous 2-photon and 3-photon multimodal signal acquisition for multiphoton microscopy

Wentao Wu, Christoph Brandt, Shuo Tang

Author Affiliations +

Proceedings Volume 12384, Multiphoton Microscopy in the Biomedical Sciences XXIII; 123840H (2023) https://doi.org/10.1117/12.2649196
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

We report on a simultaneous 2-photon and 3-photon signal acquisition method for label-free multiphoton microscopy. With dual excitation wavelengths of 1580 nm and 790 nm and a set of filter designs, multiple contrasts including second harmonic generation, third harmonic generation, and two-photon-excitation-fluorescence images are detected and separated. The spectrum of the nonlinear signals is measured and verified by a spectrometer. Depth-resolved multimodal images are demonstrated on a silicon photonic chip and leaf samples. The simultaneous 2- and 3-photon microscopy is shown to have great potential for label-free in-vivo imaging.

Conference Presentation

Citation Download Citation

Wentao Wu, Christoph Brandt, and Shuo Tang "Simultaneous 2-photon and 3-photon multimodal signal acquisition for multiphoton microscopy", Proc. SPIE 12384, Multiphoton Microscopy in the Biomedical Sciences XXIII, 123840H (25 April 2023); https://doi.org/10.1117/12.2649196

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

;

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE