LPS-induced inflammation alters cerebral oxygenation but not blood flow in a mouse model of Alzheimer’s disease

Chang Liu; Mohammed Alfadhel; Shayna Teitelbaum; Austin Birmingham; Alfredo Cardenas-Rivera; Mohammed A. Yaseen

doi:10.1117/12.2647187

25 April 2023 LPS-induced inflammation alters cerebral oxygenation but not blood flow in a mouse model of Alzheimer’s disease

Chang Liu, Mohammed Alfadhel, Shayna Teitelbaum, Austin Birmingham, Alfredo Cardenas-Rivera, Mohammed A. Yaseen

Author Affiliations +

Proceedings Volume 12384, Multiphoton Microscopy in the Biomedical Sciences XXIII; 123840L (2023) https://doi.org/10.1117/12.2647187
Event: SPIE BiOS, 2023, San Francisco, California, United States

Conference Poster

Abstract

Chronic inflammation is one of the most prominent features of Alzheimer's disease (AD). Little is known about how brain hemodynamics and oxygenation are affected by AD-related inflammation. Here, we use two-photon phosphorescence lifetime imaging with an oxygen-sensitive dye “Oxyphor 2P” to measure the partial pressure of oxygen (pO2) before and during endotoxin-induced neuroinflammation in cortical vessels of a mouse model of AD. Capillary red blood cell flux (RBC flux) was measured through two-photon phosphorescence intensity microscopy. To induce chronic inflammation, we injected lipopolysaccharide (LPS) intraperitoneally, daily for two weeks, in female APPswe: PS1dE9 mice and age-matched wild-type (WT) controls. Intravascular pO2 and RBC flux were measured in the somatosensory cortex before the LPS injection, on week 1 (day 7), and week 2 (day 15) during the LPS injection. Our results demonstrate that LPS-induced systemic inflammation leads to significant decreases in cortical intravascular pO2 while showing a negligible effect on capillary RBC flux. Moreover, AD mice are more susceptible to inflammation with more pronounced cortical pO2 reduction in comparison to WT mice. Our findings suggest that inflammation plays a key role in AD-related disruptions of cerebral tissue metabolism of oxygen.

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Chang Liu, Mohammed Alfadhel, Shayna Teitelbaum, Austin Birmingham, Alfredo Cardenas-Rivera, and Mohammed A. Yaseen "LPS-induced inflammation alters cerebral oxygenation but not blood flow in a mouse model of Alzheimer’s disease", Proc. SPIE 12384, Multiphoton Microscopy in the Biomedical Sciences XXIII, 123840L (25 April 2023); https://doi.org/10.1117/12.2647187

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