Rapid, sensitive detection of intact SARS-CoV-2 using DNA nets and a smartphone-linked fluorimeter

Hankeun Lee; Weijing Wang; Neha Chauhan; Yanyu Xiong; Nicholas Magazine; Owen Valdescruz; Dong Yeun Kim; Tianjie Qiu; Weishan Huang; Xing Wang; Brian T. Cunningham

doi:10.1117/12.2650836

16 March 2023 Rapid, sensitive detection of intact SARS-CoV-2 using DNA nets and a smartphone-linked fluorimeter

Hankeun Lee, Weijing Wang, Neha Chauhan, Yanyu Xiong, Nicholas Magazine, Owen Valdescruz, Dong Yeun Kim, Tianjie Qiu, Weishan Huang, Xing Wang, Brian T. Cunningham

Author Affiliations +

Proceedings Volume 12387, Optical Diagnostics and Sensing XXIII: Toward Point-of-Care Diagnostics; 123870B (2023) https://doi.org/10.1117/12.2650836
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

We report a single-step, room-temperature, five to ten minute SARS-CoV-2 saliva self-monitoring method that overcomes the limitations of existing approaches through the use of fluorophore-releasing Designer DNA Nanostructures (DDNs) that bind with the multivalent pattern of spike proteins on the exterior intact virions and an inexpensive smartphone-linked, pocket-size fluorimeter, called a “V-Pod” for its resemblance to an Apple AirPod™ headphone case. We characterize the V-Pod fluorimeter performance and the DDN-based assay to demonstrate a clinically relevant detection limit of 10⁴ virus particles/mL for pseudo-typed WT SARS-CoV-2 and 10⁵ virus particles/mL for real pathogenic variants, including Delta, Omicron, and D614g.

Conference Presentation

Citation Download Citation

Hankeun Lee, Weijing Wang, Neha Chauhan, Yanyu Xiong, Nicholas Magazine, Owen Valdescruz, Dong Yeun Kim, Tianjie Qiu, Weishan Huang, Xing Wang, and Brian T. Cunningham "Rapid, sensitive detection of intact SARS-CoV-2 using DNA nets and a smartphone-linked fluorimeter", Proc. SPIE 12387, Optical Diagnostics and Sensing XXIII: Toward Point-of-Care Diagnostics, 123870B (16 March 2023); https://doi.org/10.1117/12.2650836

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