A simple, pipette-free, and power-free device for virus nucleic acid detection

Mengdi Bao; Shuhuan Zhang; Chad ten Pas; Stephen J. Dollery; Ruth V. Bushnell; . Yuqing; Rui Liu; Guoyu Lu; Gregory J. Tobin; Ke Du

doi:10.1117/12.2653148

17 March 2023 A simple, pipette-free, and power-free device for virus nucleic acid detection

Mengdi Bao, Shuhuan Zhang, Chad ten Pas, Stephen J. Dollery, Ruth V. Bushnell, . Yuqing, Rui Liu, Guoyu Lu, Gregory J. Tobin, Ke Du

Author Affiliations +

Proceedings Volume 12397, Frontiers in Biological Detection: From Nanosensors to Systems XV; 1239705 (2023) https://doi.org/10.1117/12.2653148
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

A portable, inexpensive, and easy-to-manufacture microfluidic device is developed for the detection of SARS-CoV-2 dsDNA fragments. In this device, four reaction chambers separated by carbon fiber rods are pre-loaded with isothermal amplification and CRISPR-Cas12a reagents. The reaction is carried out by simply pulling the rods, without the need for manual pipetting. To facilitate power-free pathogen detection, the entire detection is designed to be heated with a disposable hand warmer. After the CRISPR reaction, the fluorescence signal generated by positive samples is identified by naked eye, using an inexpensive flashlight. This simple and sensitive device will serve as a new model for the next-generation viral diagnostics in either hospital or resource-limited settings.

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Mengdi Bao, Shuhuan Zhang, Chad ten Pas, Stephen J. Dollery, Ruth V. Bushnell, . Yuqing, Rui Liu, Guoyu Lu, Gregory J. Tobin, and Ke Du "A simple, pipette-free, and power-free device for virus nucleic acid detection", Proc. SPIE 12397, Frontiers in Biological Detection: From Nanosensors to Systems XV, 1239705 (17 March 2023); https://doi.org/10.1117/12.2653148

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