Determination of cysteine content by Cu(І)-neocuproine spectrophotometry

Changqing Tu; Xinrong Wen

doi:10.1117/12.2673904

4 April 2023 Determination of cysteine content by Cu(І)-neocuproine spectrophotometry

Changqing Tu, Xinrong Wen

Proceedings Volume 12603, Biophysical Society of GuangDong Province Academic Forum: Precise Photons and Life Health (PPLH 2022); 1260302 (2023) https://doi.org/10.1117/12.2673904
Event: 2022 Biophysical Society of Guang Dong Province Academic Forum - Precise Photons and Life Health (PPLH 2022), 2022, Guangzhou, China

Abstract

Cu²⁺ can be reduced to Cu⁺ by -SH (sulfhydryl) in cysteine, then neocuproine as the chromogenic reagent reacts with Cu⁺ to form the yellow complex. At the maximum absorption wavelength, the amount of the yellow complex is directly proportional to the added amount of cysteine in a certain range. According to this principle, the content of cysteine can be determined by spectrophotometry. Thus, a method for determination of cysteine by copper(I)–neocuproine spectrophotometry is established. Under the optimal conditions, this method is used to determinate the cysteine content in food, and the determination results are basically close to the result by pharmacopoeia method. The recovery rates of standard addition are 102.4%~104.3%.

Citation Download Citation

Changqing Tu and Xinrong Wen "Determination of cysteine content by Cu(І)-neocuproine spectrophotometry", Proc. SPIE 12603, Biophysical Society of GuangDong Province Academic Forum: Precise Photons and Life Health (PPLH 2022), 1260302 (4 April 2023); https://doi.org/10.1117/12.2673904

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available