Multimodal optical coherence microscopy, mechano-microscopy, and fluorescence microscopy for three-dimensional characterization of multicellular spheroids

Alireza Mowla; Matt S. Hepburn; Jiayue Li; Liisa M. Hirvonen; Danielle Vahala; Sebastian Amos; Samuel Maher; Yu Suk Choi; Brendan F. Kennedy

doi:10.1117/12.2670830

11 August 2023 Multimodal optical coherence microscopy, mechano-microscopy, and fluorescence microscopy for three-dimensional characterization of multicellular spheroids

Alireza Mowla, Matt S. Hepburn, Jiayue Li, Liisa M. Hirvonen, Danielle Vahala, Sebastian Amos, Samuel Maher, Yu Suk Choi, Brendan F. Kennedy

Author Affiliations +

Proceedings Volume 12632, Optical Coherence Imaging Techniques and Imaging in Scattering Media V; 1263219 (2023) https://doi.org/10.1117/12.2670830
Event: European Conferences on Biomedical Optics, 2023, Munich, Germany

Abstract

Multicellular spheroids are a powerful model to study biochemical and biophysical interactions between cancer cells during growth and progression. However, little is known about how the biomechanics of the three-dimensional (3-D) microenvironment control cancer cell behaviors due to the lack of enabling technologies that can measure 3-D subcellular-scale elasticity and co-register it with the morphology and function of cells in a 3-D microenvironment. Here, we propose a multimodal imaging system that integrates an optical coherence microscopy-based subcellular mechano-microscopy system with a multi-channel confocal fluorescence microscopy system. Using this multimodal imaging system, we scan non-metastatic MCF7 breast cancer cell spheroids encapsulated in gelatin methacryloyl (GelMA) hydrogels and co-register 3-D intra-spheroid elasticity with subcellular structures, such as nuclei and cell membranes.

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Alireza Mowla, Matt S. Hepburn, Jiayue Li, Liisa M. Hirvonen, Danielle Vahala, Sebastian Amos, Samuel Maher, Yu Suk Choi, and Brendan F. Kennedy "Multimodal optical coherence microscopy, mechano-microscopy, and fluorescence microscopy for three-dimensional characterization of multicellular spheroids", Proc. SPIE 12632, Optical Coherence Imaging Techniques and Imaging in Scattering Media V, 1263219 (11 August 2023); https://doi.org/10.1117/12.2670830

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KEYWORDS

3D scanning

Fluorescence microscopy

Optical coherence microscopy

Fluorescence

Beam splitters

Compliance

Elasticity

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