High-resolution imaging through scattering media by integration of wavefront shaping and imaging scanning microscopy

Tong Peng; Runze Li; Dan Dan; Chen Bai; Junwei Min; Meiling Zhou; Baoli Yao

doi:10.1117/12.2686630

29 November 2023 High-resolution imaging through scattering media by integration of wavefront shaping and imaging scanning microscopy

Tong Peng, Runze Li, Dan Dan, Chen Bai, Junwei Min, Meiling Zhou, Baoli Yao

Author Affiliations +

Proceedings Volume 12766, Advanced Optical Imaging Technologies VI; 127660V (2023) https://doi.org/10.1117/12.2686630
Event: SPIE/COS Photonics Asia, 2023, Beijing, China

Conference Poster

Abstract

Optical microscopy is an indispensable imaging tool in life science. However, light cannot be focused owing to high scattering, and hence, the imaging depth and spatial resolution are restricted. Here, we propose an imaging method that combines wavefront shaping and image scanning microscopy. The reflected signal is used as feedback to acquire an optimal phase that can refocus the scattered light behind the scattering media. The experimental results show that the proposed method works in multilayer scattering media and can improve both the resolution and imaging depth of optical microscopy.

Citation Download Citation

Tong Peng, Runze Li, Dan Dan, Chen Bai, Junwei Min, Meiling Zhou, and Baoli Yao "High-resolution imaging through scattering media by integration of wavefront shaping and imaging scanning microscopy", Proc. SPIE 12766, Advanced Optical Imaging Technologies VI, 127660V (29 November 2023); https://doi.org/10.1117/12.2686630

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