High-throughput and label-free cell cluster monitoring based on light scattering imaging and microfluidic technique

Ning Zhang; Rui Xu; Yawei Li; Weiwei Chen; Qiongqiong Ren; Linyan Xie

doi:10.1117/12.2688990

27 November 2023 High-throughput and label-free cell cluster monitoring based on light scattering imaging and microfluidic technique

Ning Zhang, Rui Xu, Yawei Li, Weiwei Chen, Qiongqiong Ren, Linyan Xie

Author Affiliations +

Proceedings Volume 12770, Optics in Health Care and Biomedical Optics XIII; 127703B (2023) https://doi.org/10.1117/12.2688990
Event: SPIE/COS Photonics Asia, 2023, Beijing, China

Conference Poster

Abstract

Efficient detection of cell clusters is of paramount importance in the production and packaging processes of single-cell suspensions, as it can significantly impact cell density uniformity, induce apoptosis, and compromise the accuracy of cell sorting outcomes. This study introduces a novel, high-throughput and label-free method for monitoring cell clusters, leveraging light scattering imaging and microfluidic technologies. Two-dimensional (2D) light scattering, as a vital label free analytical approach, proves effective in detecting and analyzing biological particles with intricate structures. Through optical microscopy coupled with a high-speed camera, this study examines the 2D light scattering patterns produced by cell clusters, facilitating the discrimination between single cells and clustered cells. This label-free methodology offers distinct advantages over traditional labeling techniques, as it preserves cellular integrity without invasive disruptions. Furthermore, innovative microfluidic chip design enables continuous real-time monitoring of cell clusters, empowering rapid and high-throughput detection. Experimental validation involved monitoring silicon oxide (SiO₂) microsphere suspensions, demonstrating the method's capacity for high-throughput and high-sensitivity cell cluster monitoring. This research presents a promising tool for efficiently handling and monitoring the production of single-cell suspensions, with potential applications in various fields of cell biology and biotechnology.

(2023) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Ning Zhang, Rui Xu, Yawei Li, Weiwei Chen, Qiongqiong Ren, and Linyan Xie "High-throughput and label-free cell cluster monitoring based on light scattering imaging and microfluidic technique", Proc. SPIE 12770, Optics in Health Care and Biomedical Optics XIII, 127703B (27 November 2023); https://doi.org/10.1117/12.2688990

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