Multimodal metabolic imaging of the ovary under diet manipulation during aging process

Yajuan Li; Hongje Jang; Zhi Li; Yuhan Li; Lingyan Shi

doi:10.1117/12.3006234

12 March 2024 Multimodal metabolic imaging of the ovary under diet manipulation during aging process

Yajuan Li, Hongje Jang, Zhi Li, Yuhan Li, Lingyan Shi

Proceedings Volume 12847, Multiphoton Microscopy in the Biomedical Sciences XXIV; 128470E (2024) https://doi.org/10.1117/12.3006234
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Lipid and protein metabolism play important roles in the aging process of ovary. High resolution in situ optical imaging provides a powerful approach to study the metabolic dynamics of macro-molecules such as lipids and proteins. Here, we applied a multimodal imaging platform integrated with D2O probing stimulated Raman scattering (DO-SRS), multiphoton fluorescence (MPF), and second harmonic generation (SHG) to explore metabolic changes of biomolecules in Drosophila ovaries during aging process. In this study, the sub-cellular spatial distribution of de novo protein synthesis, lipogenesis and redox ratio in ovaries are quantitatively imaged and examined in different ages. The regulation of diets on aging-dependant changes of ovary metabolism are investigated. Our results provide valuable insights for the underlying mechanism of ovary aging and how to intervene the aging process to achieve a better health

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Yajuan Li, Hongje Jang, Zhi Li, Yuhan Li, and Lingyan Shi "Multimodal metabolic imaging of the ovary under diet manipulation during aging process", Proc. SPIE 12847, Multiphoton Microscopy in the Biomedical Sciences XXIV, 128470E (12 March 2024); https://doi.org/10.1117/12.3006234

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available