A multi-colour 2D and 3D structured illumination microscope using MEMS scanning mirrors

Peter W. Tinning; Jay Christopher; Mark Donnachie; Deepak Uttamchandani; Ralf Bauer

doi:10.1117/12.3002511

12 March 2024 A multi-colour 2D and 3D structured illumination microscope using MEMS scanning mirrors

Peter W. Tinning, Jay Christopher, Mark Donnachie, Deepak Uttamchandani, Ralf Bauer

Proceedings Volume 12849, Single Molecule Spectroscopy and Superresolution Imaging XVII; 1284902 (2024) https://doi.org/10.1117/12.3002511
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

We present our latest results on a structured illumination microscope (SIM) implementation using individual microelectromechanical systems (MEMS) micromirrors with three-axis full angular, radial and phase control of the illumination pattern in the sample. Results of a simultaneous multi-color 2D SIM and 3D SIM implementation are shown with digital system adjustment to select the optimal imaging conditions and adapt to variable microscope objectives used in the system. Calibration and cell images of 2D and 3D samples will be shown to verify the resolution enhancement and axial sectioning potential.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Peter W. Tinning, Jay Christopher, Mark Donnachie, Deepak Uttamchandani, and Ralf Bauer "A multi-colour 2D and 3D structured illumination microscope using MEMS scanning mirrors", Proc. SPIE 12849, Single Molecule Spectroscopy and Superresolution Imaging XVII, 1284902 (12 March 2024); https://doi.org/10.1117/12.3002511

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KEYWORDS

Light sources and illumination

Microelectromechanical systems

Mirrors

3D image processing

Microscopes

Imaging systems

Micromirrors

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