Quantitative study of phase sensitivity in immersion-based lensless digital holographic microscopy

E. Wdowiak; M. Rogalski; M. Trusiak

doi:10.1117/12.3008488

12 March 2024 Quantitative study of phase sensitivity in immersion-based lensless digital holographic microscopy

E. Wdowiak, M. Rogalski, M. Trusiak

Proceedings Volume 12852, Quantitative Phase Imaging X; 128520M (2024) https://doi.org/10.1117/12.3008488
Event: SPIE BiOS, 2024, San Francisco, California, United States

Conference Poster

Abstract

This study introduces a methodology for quantitative assessment of phase measurement sensitivity in lensless digital holographic microscopy (LDHM) setups, incorporating an immersion medium between the object and the detector. Utilizing two setup configurations, we systematically investigated the influence of immersion conditions on phase accuracy, numerical reconstruction, and twin-image artefacts. Employing the Angular Spectrum and iterative Gerchberg-Saxton methods, we reconstructed phase maps for varying immersion thicknesses. Results demonstrate that immersion medium has minimal influence on phase sensitivity but significantly reduces twin-image artifacts when in direct contact with the object, providing valuable insights for developing LDHM biological applications.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

E. Wdowiak, M. Rogalski, and M. Trusiak "Quantitative study of phase sensitivity in immersion-based lensless digital holographic microscopy", Proc. SPIE 12852, Quantitative Phase Imaging X, 128520M (12 March 2024); https://doi.org/10.1117/12.3008488

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