Quantified interface chemistry and biophysics via water imaging

S. Roke

doi:10.1117/12.3003675

12 March 2024 Quantified interface chemistry and biophysics via water imaging

S. Roke

Author Affiliations +

Proceedings Volume 12855, Advanced Chemical Microscopy for Life Science and Translational Medicine 2024; 1285506 (2024) https://doi.org/10.1117/12.3003675
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

High throughput wide-field second harmonic imaging enables the label-free imaging of interfacial (< 3 nm thick) water, with a spatial resolution of ~370 nm using ~100 ms acquisition times per image. The obtained interfacial orientational order of water can be used to create spatiotemporal transmembrane potential maps of free-standing lipid membranes, giant unilamellar vesicles or living cells. These maps are then used to uniquely quantify membrane-water interactions, which show surprisingly heterogeneous behavior which sheds new light on processes such as ion transport, ion channel operation and membrane deformation.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

S. Roke "Quantified interface chemistry and biophysics via water imaging", Proc. SPIE 12855, Advanced Chemical Microscopy for Life Science and Translational Medicine 2024, 1285506 (12 March 2024); https://doi.org/10.1117/12.3003675

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