Impulsive stimulated Raman scattering imaging using an ultra-fast acoustic-optics delay line

Sisira Suresh; Ines Martin; Samuel Metais; Siddarth Shivkumar; Nicolas Forget; Randy Bartels; Herve Rigneault

doi:10.1117/12.2692912

12 March 2024 Impulsive stimulated Raman scattering imaging using an ultra-fast acoustic-optics delay line

Sisira Suresh, Ines Martin, Samuel Metais, Siddarth Shivkumar, Nicolas Forget, Randy Bartels, Herve Rigneault

Proceedings Volume 12855, Advanced Chemical Microscopy for Life Science and Translational Medicine 2024; 1285507 (2024) https://doi.org/10.1117/12.2692912
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

vibrational motion of a molecule is intrinsically linked to its structure and composition, which provides a way to identify it. Raman spectroscopy, utilizing the inelastic scattering of light, investigates these molecular vibrations. While Raman shifts exceeding 200 cm⁻¹ primarily capture intramolecular vibrations, lower Raman shifts ( < 200 cm⁻¹) provide insights into the collective motion of molecules, thereby revealing valuable structural information. Although frequency domain imaging effectively addresses higher Raman shifts, a time domain approach proves more practical for lower Raman shifts. Impulsive Stimulated Raman Scattering (ISRS) is a time domain technique that employs a pump pulse to instantaneously excite a molecule, activating all modes within its bandwidth and inducing a transient refractive index modulation. This modulation can be probed by a second pulse, enabling analysis of spatial profile, spectrum, and polarization changes. In this study, we elucidate the implementation of transient vibrational refractive index detection for the acquisition of ultrafast hyperspectral images, including the integration of a random access delay line into the existing setup that enables scanning windows of up to 50 picoseconds at random time delays.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Sisira Suresh, Ines Martin, Samuel Metais, Siddarth Shivkumar, Nicolas Forget, Randy Bartels, and Herve Rigneault "Impulsive stimulated Raman scattering imaging using an ultra-fast acoustic-optics delay line", Proc. SPIE 12855, Advanced Chemical Microscopy for Life Science and Translational Medicine 2024, 1285507 (12 March 2024); https://doi.org/10.1117/12.2692912

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