Ultrafast studies on a cyanobacterial chloride pump and its proton pumping mutant

Harmanjot S. Grewal; Jessica Besaw; Soumyajit Mitra; Manoel L. da Silva-Neto; R. J. D. Miller

doi:10.1117/12.3001901

12 March 2024 Ultrafast studies on a cyanobacterial chloride pump and its proton pumping mutant

Harmanjot S. Grewal, Jessica Besaw, Soumyajit Mitra, Manoel L. da Silva-Neto, R. J. D. Miller

Proceedings Volume 12875, Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XXIV; 128750I (2024) https://doi.org/10.1117/12.3001901
Event: SPIE LASE, 2024, San Francisco, California, United States

Conference Poster

Abstract

Mastigocladopsis repens Rhodopsin (MastR), the newest member of a family of retinal-binding anionic transmembrane pumps, can trap light energy to allow inward chloride transport across a cellular membrane. The pumping action is driven by retinal isomerization, which is thermodynamically unfavorable in the ground state but occurs at a higher rate under photo-excitation in the host protein environment. Particularly interesting is MastR’s conversion to an outward proton pump (MastR-T74D) due to single-point mutation of an amino acid located close to the retinal chromophore. Although flash photolysis studies have characterized the MastR/MastR-T74D photocycle on a microsecond timescale, its ultrafast dynamics involving retinal isomerization have not yet been investigated. We performed ultrafast transient absorption studies on MastR and MastR-T74D to look at the femtosecond to picosecond dynamics that lead to retinal isomerization and compared them to the well-studied ultrafast dynamics of bacteriorhodopsin.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Harmanjot S. Grewal, Jessica Besaw, Soumyajit Mitra, Manoel L. da Silva-Neto, and R. J. D. Miller "Ultrafast studies on a cyanobacterial chloride pump and its proton pumping mutant", Proc. SPIE 12875, Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XXIV, 128750I (12 March 2024); https://doi.org/10.1117/12.3001901

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