In this study we have developed a compact and versatile phase camera functioning as a wavefront sensor for macroscopy or microscopy applications. This device records two intensity images at different focal points and, with the integration of an electrically tunable lens (ETL), operates in real time. Working with intensity images allows achieving high resolutions, near the actual CCD/CMOS sensor resolution. Here we show the application of the camera in two very different scenarios, a macroscopic application, where the camera was coupled with a simple lens relay to study the behavior of a deformable mirror (DM); and characterize defocus and astigmatism in optical lenses. On the second example, the camera was attached directly to a microscope using a simple c-mount to follow human blood moving in real time.
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