Second harmonic generation microscopy: experiments and theory

Albert Suceava; Akash Saha; Venkatraman Gopalan

doi:10.1117/12.3029590

3 October 2024 Second harmonic generation microscopy: experiments and theory

Albert Suceava, Akash Saha, Venkatraman Gopalan

Proceedings Volume 13139, Ultrafast Nonlinear Imaging and Spectroscopy XII; 1313909 (2024) https://doi.org/10.1117/12.3029590
Event: Optical Engineering + Applications, 2024, San Diego, California, United States

Abstract

Second harmonic generation (SHG) microscopy, involving the doubling of the frequency of light by a material, is a valuable characterization technique that is highly sensitive to local material symmetry at lengths scales close to the diffraction limit. The use of high-NA microscope objectives introduces an additional layer of complexity when performing quantitative analysis of SHG polarimetry data due to the appreciable effects that strong focusing has on the polarization of the probe beam. A systematic investigation of this problem is presented, producing analytical and numerical solutions of SHG polarimetry generated under high-NA lenses in a microscope setup. Modeling of a variety of standard samples, from single crystals to thin films, is performed and compared against experimental data.

Conference Presentation

Citation Download Citation

Albert Suceava, Akash Saha, and Venkatraman Gopalan "Second harmonic generation microscopy: experiments and theory", Proc. SPIE 13139, Ultrafast Nonlinear Imaging and Spectroscopy XII, 1313909 (3 October 2024); https://doi.org/10.1117/12.3029590

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