Time-resolved mid-infrared photothermal microscopy for heat transfer dynamics across aqueous interfaces

Panagis Samolis; Poyraz Durgun; Michelle Y. Sander

doi:10.1117/12.3028230

3 October 2024 Time-resolved mid-infrared photothermal microscopy for heat transfer dynamics across aqueous interfaces

Panagis Samolis, Poyraz Durgun, Michelle Y. Sander

Proceedings Volume 13139, Ultrafast Nonlinear Imaging and Spectroscopy XII; 131390M (2024) https://doi.org/10.1117/12.3028230
Event: Optical Engineering + Applications, 2024, San Diego, California, United States

Abstract

Photothermal imaging has proven a powerful label-free chemical imaging technique. With time-resolved mid-infrared photothermal imaging heat transfer dynamics across aqueous interfaces can be studied. However, liquid water has been a limiting factor in mid-infrared imaging and spectroscopy due to its high absorption spanning across the molecular fingerprint region so that cellular imaging is often performed in less absorbing heavy water instead. Time-resolved measurements via boxcar detection enable the separation of water background and reveal how heat transfer dynamics across aqueous interfaces strongly depend on hydration and the surrounding environment. Mid-infrared photothermal imaging of extracted axon-bundles from crayfish is presented in a saline solution where the water background can be separated based on its inherently different transient response.

Conference Presentation

Citation Download Citation

Panagis Samolis, Poyraz Durgun, and Michelle Y. Sander "Time-resolved mid-infrared photothermal microscopy for heat transfer dynamics across aqueous interfaces", Proc. SPIE 13139, Ultrafast Nonlinear Imaging and Spectroscopy XII, 131390M (3 October 2024); https://doi.org/10.1117/12.3028230

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